TIE UP SIN app

Which data input report format & filters?

Spectronaut settings prop.

Spectronaut Report scheme

Spectronaut: Bait : Bait

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Spectronaut: Bait : WT

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Spectronaut: WT : WT

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DIA-NN settings prop.

DIA-NN: Bait : Bait

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DIA-NN: Bait : WT

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DIA-NN: WT : WT

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DIA-NN: fasta file(s) used for the analysis

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Q-value

ion CV

min. peptides

sequence coverage [%]

control FC threshold

statistics Q-value threshold

statistics fold-change threshold

TIE UP SIN app version 1.0.0

please cite:

Schedlowski, M. et al. TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions. Front. Microbiol. 16, 1657647 (2025).